| Title | [Influence of As(2)O(3) on Proteasome beta(1)-subunit in NB4 Cells.] | | Author(s) | Lü XH, Chen Y, Zhang M, He PC, Wang HY, Ni ZF, Lu R | | Institution | Department of Rheumatology, The First Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an 710061, Shaanxi Province, China. | | Source | Zhongguo Shi Yan Xue Ye Xue Za Zhi 2009 Jun; 17(3):579-82. | | Abstract | This study was aimed to expolore the correlation between effects of arsenic trioxide on NB4 cell differentiation and the change of beta(1)-subunit of 26S proteasome. NB4 cell in 0.5 micromol/L As(2)O(3) was incubated for 24 hours and 48 hours, then total protein was extracted, expressions of subunit beta(1) and PML-RARalpha fusion protein were determined by Western blot. The results indicated that the expression of 26S proteasome beta(1)-subunit increased after incubation with As(2)O(3) for 24 hours, but after culture with As(2)O(3) for 48 hours, the expression of beta-subunit decreased to the baseline. Meanwhile, the expression of PML-RARalpha fusion protein obviously decreased after 24 hours, and kept low level at 48 hours. It is concluded that the expression of 26S proteasome beta(1)-subunit increases after exposure to As(2)O(3). Increment of 26S proteasome beta(1)-subunit may be associated with the degradation of PML-RARalpha fusion protein and plays roles in the differentiation and apoptosis of NB4 cells. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 19549367 |
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